Abstract
UV exposure is harmful to plants. Increasing resistance against UV light is thus of great importance to their growth. Para-Aminobenzoic acid (PABA) has major roles in many biological processes, involving nucleotide biosynthesis, DNA repair, and DNA methylation, which contributed to UV irradiation. However, no study reports the effect of PABA on UV tolerance, or details of the underlying molecular mechanisms are explored. Hence, the objective of the research is to study the protective effect of PABA on UV in Arabidopsis and explored the molecular mechanisms. We overexpressed PABA synthase gene (Pabs) from Mushroom Agaricus bisporus in Arabidopsis and observed reduced root growth and UV-C hyposensitivity exposed to 2500 J m−2 UV-C light. UV-C-induced DNA damage was significantly reduced and the expression of decreased DNA methylation 1 (DDM1) was remarkably higher in the Pabs lines, suggesting that overexpression of Pabs may protect against UV-induced DNA damage. In addition, overexpression of Pabs leads to an elevated reactive oxygen species production at root tips and enhanced catalase and superoxide dismutase activity, which may correlate with the enhanced UV tolerance of the Pabs overexpression lines. In summary, overexpression of Pabs from A. bisporus enhances UV-C tolerance of Arabidopsis, suggesting that Pabs takes an important part in defence against DNA damage.
Highlights
Light is crucial to plant growth and development
The roles of PABA synthase gene (Pabs) in UV resistance were investigated in Pabs transgenic Arabidopsis plants
Pabs overexpressing Arabidopsis was less susceptible to UV-C than Col proved by relative primary root elongation assay and the morphogenic responses
Summary
Light is crucial to plant growth and development. elevated ultraviolet irradiation (UV, 100–290 nm) has detrimental or mutagenic effects on plants, which sometimes can even be lethal (Bray and West 2005; Suchar and Robberecht 2015). UV light can damage plant proteins, lipids, DNA and affect plant development, including inhibition of photosynthesis, increases in ROS, peroxidation of lipids, alteration of metabolism and gene expression (Abdel et al 2013; Caverzan et al 2016; Gill et al 2015; Robson et al 2015; Suchar and Robberecht 2016). These can result in changes in leaf size, root growth, and apoptotic-like effects (Lee and Suh 2015; Migicovsky and Kovalchuk 2014; Verdaguer et al 2017). They may reduce the penetration of UV light by means of physiological changes such as alternations in epicuticular waxes (Lee and Suh 2015), production of
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