Abstract
Hu sheep (Ovis aries) is a rare white sheep breed, with four different types of lambskin patterns that have different values. However, the genetic mechanisms underlying different types of pattern formation remains unclear. This research aimed to characterize the molecular mechanism of differentially expressed gene PAPPA2 affecting the pattern type of Hu sheep’s lambskin at the cellular level. Thus, RT-qPCR, EdU and Cell Cycle detection were used to explore the effect of PAPPA2 and IGFBP5 (a protein that can be hydrolyzed by PAPPA2) on the proliferation of dermal papilla cells (DPCs) after overexpression or interference with PAPPA2 and IGFBP5. The expression level of PAPPA2 in straight DPCs was 4.79 ± 1.84 times higher than curved. Overexpression of PAPPA2 promoted the proliferation of DPCs and also increased the expression of IGFBP5. Conversely, overexpression of IGFBP5 reduced the proliferation of DPCs. However, the proliferation of DPCs was restored by co-overexpression of PAPPA2 and IGFBP5 compared with overexpression of IGFBP5 alone. Thus, PAPPA2 can affect the proliferation of DPCs through regulating IGFBP5 and then participate in lambskin pattern determination. Overall, we preliminarily clarified the critical role played by PAPPA2 during the formation of different pattern in Hu sheep lambskin.
Highlights
Hu sheep, native to China, is a rare white lambskin breed, its lambskin has four pattern types: extremely curved, medium curved, little curved and straight [1] (Figure 1)
When the embryos continues to grow to 120d fetal age, a wave pattern will form with proper hair length, but as the embryos continue to grow (140 d fetal age), the wave pattern in some of the embryos will gradually disappear and change to straight hair because of the excessive length of the fetal hair [2]
dermal papilla (DP) plays an important role in the regulation of both hair follicle development and cycle [6]
Summary
Native to China, is a rare white lambskin breed, its lambskin has four pattern types: extremely curved (named “Curved” in this article), medium curved, little curved and straight [1] (named “Straight” in this article) (Figure 1). The formation of different pattern types is believed to be related to the length of fetal hair; at the beginning (110 d fetal age), the fetal hair just starts to grow. The growth of wool is closely related to the development of the hair follicle (HF). DP plays an important role in the regulation of both hair follicle development and cycle [6]
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