Abstract
A method has been described for separation and quantitative estimation of creatine and creatinine in human plasma by paper chromatography. The 2 amines were adsorbed on Dowex 50, eluted with triethylamine and separated by subsequent paper chromatography in n-butanol-acetic acid-pyridine-water (4:1:1:2). Creatine was converted to creatinine by spraying the paper with glacial acetic acid and subsequent heating. After detection with Jaffé reagent the spots were eluted and the colour intensity was measured photometrically. By this method the following average values of plasma creatinine were found: males (10 blood donors) : 0.78 ± 0.10 mg%; females (10 blood donors): 0.64 ± 0.08 mg%. The corresponding values for plasma creatine were 0.46 ± 0.10 mg% (males) and 0.91 ± 0.28 mg% (females).
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