Abstract
To obtain healthy and good quality plants from in vitro cultivation, it is necessary to produce plantlets with well-developed rooting systems because they must undergo acclimatization, a final and a very difficult stage of micropropagation. In the present research, the effect of auxins NAA, IAA and IBA in concentrations of 0.5; 1; 2.5 and 5 mg·dm−3 on the Paphiopedilum insigne in vitro rooting was studied, and it was noted that 1 mg·dm−3 of IAA or IBA enabled the obtaining of a lot of rooted and good quality plantlets. The subsequent influence of the two most advantageous auxins on the acclimatization of plantlets in different substrates (sphagnum moss, sphagnum moss + substrate for orchids, substrate for orchids, substrate for orchids + acid peat) was tested, in the means of morphological features of plants and their physiological parameters, i.e., chlorophyll fluorescence (FV, Fm, Fv/Fm), stress enzyme activity (catalase, ascorbate peroxidase), and water balance. Considering all the tested features, it might be stated that the best results were obtained when explants were rooted in vitro in the presence of 1 mg·dm−3 of IAA and then planted ex vitro in substrate for orchids.
Highlights
The media were supplemented with auxins: 1-naphthaleneacetic acid (NAA), indolyl-3-acetic acid (IAA) or indolyl-3-butyric acid (IBA) in concentrations of
Media supplemented with 1 mg·dm−3 indole3-butyric acid (IBA) enabled the obtaining of 100% of rooted plants in vitro with the largest number and longest roots
Taking into consideration both the morphological features of seedlings and the rooting, indole-3-acetic acid (IAA) in a concentration of 1 mg·dm−3 was proved to be the most advantageous auxin to be used in the rooting of
Summary
Ex Lindl.) Pfitzer (Orchidaceae), a so-called slipper orchid, is a valued pot plant that attracts orchid lovers with original flowers with lips similar to a shoe. It is used in floral compositions as a cut flower. A rooting system is necessary to allow water and nutrient intake by plants and to maintain the plants stably in soil [4]. These problems can usually be successfully solved by the use of appropriate plant hormones, mainly auxins [5]
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