Abstract
Escherichia coli O157:H7 (E. coli O157:H7) is a typical foodborne pathogen that contaminates food and water, leading to lots of infectious diseases. In this study, a near-infrared-responsive photoelectrochemical (PEC) sensing platform for the ultrasensitive detection of E. coli O157:H7 was established by assembling a flexible conductive paper electrode with core-shell-structured upconversion nanophosphors (UCNPs) @SiO2@Ag and carbon self-doped graphitic carbon nitride (C-g-C3N4). In this design, UCNPs act as a self-powered that change near infrared (NIR) excitation into visible emission, while the semiconductor material C-g-C3N4 acts as the energy-conversion center to convert visible illumination to photocurrent. Importantly, silver nanoparticles (AgNPs) significantly enhance the upconversion luminescence of UCNPs and facilitate the separation and transportation of photoelectrons by the localized surface plasmon resonance effect. Using the antimicrobial peptide Magainin I as the recognition element, E. coli O157:H7 was specifically captured and determined directly for its steric hindrance effect on the photoelectrode. As a result, the photocurrent decreased linearly with the increase in E. coli O157:H7 in a wide range from 5 to 5 × 106 colony-forming units per mL (CFU/mL), and the detection limit was as low as 2 CFU/mL. Finally, the proposed NIR PEC sensor was successfully utilized for the determination of E. coli O157:H7 in contaminated pork, cabbage, and milk samples without enrichment of the bacteria in 50 min.
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