Abstract
Gas chromatography (GC) and thin-layer chromatography (TLC) have received most attention as techniques for separation of stereoisomers. Indirect separations by GC of diastereomers have been extensively reviewed by Gil-Av and Nurok [1] while Lochmuller and Souter recently reviewed direct GC resolution methods [2]. TLC work involving diastereomeric compounds of various classes: β-methyltryptophans [3], di- and tripeptides [4], some aliphatic compounds [5], and thymidine hydrates [6] have been reported. Although much attention is given to diastereomer separations by GC and TLC, no reports exist of such applications by electrophoresis. This report describes some high voltage electrophoretic separations of diastereomers of several pharmaceutically and biochemically useful compounds.
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