ウンカ類の生態識別に関するpaper chromatographyの応用

Abstract

The present study was undertaken to clarify that the paper chromatography on free amino acids can be successfully applied to the discrimination of individuality in the developmental stages of the brown planthoppers, Nilaparvata lugens STAL.Paper chromatographic method was applied to whole specimens of insect, and one-or twodimensional chromatograms were obtained and qualitative and quantitative differences of individualities were revealed by their respective. patterns of ninhidrin-positive substances. Thus obtained results were concluded as follows.1. Though the one-dimensional chromatograms of the 1 st to 3 rd instar nymphs were easily obtained, two-dimensional ones of them were very difficult because of their small quantities of free amino acids.2. From the qualitative and quantitative comparisons of two-dimensional chromatographic patterns, three groups of amino acids in 5th instar nymphs, two groups in macropterous females, three groups in brachypterous males and two groups in macropterous males were clearly recognized, respectively.3. Sensitivities of ninhidrin-reaction of specimens which were reared by mature leaves of rice plant were superior to those of specimens reared by young rice plants. And reactions in autumn generation were far more sensitive than those in summer generation.Thus, it may be considered that the paper chromatography can be employed as a comparative tool to whole insect specimens.