Paper chromatographic separation and identification of phenol derivatives and related compounds of biochemical interest using a reference system: Fifth supplement

Abstract

Following previous supplements in this series, a further 180 compounds were analyzed by paper chromatography in six solvent systems. The data originating from 15 standard colour reagents are also recorded and presented. The following types of compounds are described: flavonoid derivatives, aliphatic and aromatic aldoximes and ketoximes, substituted benzamide derivatives, aminobenzene derivatives and heterocyclic amines, N-nitroso compounds, N-oxides of tertiary bases, pyrimidine and indole derivatives and sulphonamide drugs. The paper chromatographic mobility patterns were characteristic, especially for 1,3- and 1,4-hydroxybenzaldoximes and ketoximes of the hydroxyacetophenone type. All the heterocyclic bases investigated showed mobility patterns similar to those of the alkaloids described in earlier supplements. Mobility patterns similar to the alkaloids were produced by some of the mercaptopyrimidines. In general amino-, diamino-, hydroxy and dihydroxy derivatives of pyrimidines showed low mobility in these solvents. Several flavonoid compounds were found to be Erhlich positive and a magenta colour was produced by l-epicatechin. Most of the N-unsubstituted benzamides were detected with p-dimethylaminocinnamaldehyde, and most of the N-nitroso derivatives of secondary amines and N-oxides of heterocyclic tertiary amines were revealed by this reagent.