Abstract
Abstract A paper chromatographic method is described for determination of pyridine and a-picoline in ordinary denatured spirit, french polish, and their derived illicit liquors. The method is based on conversion of the pyridine bases to their salts (sulfate or hydrochloride). The salt residue is subjected to 2-dimensional paper chromatography on filter paper, using methanol-butanol-acetic acid-ethyl acetate (20 + 10 + 1 + 10) as developing solvents, and is sprayed uniformly with sodium rhodizonate followed by barium chloride (for sulfate salts), or ammmoniacal silver nitrate followed by fluorescein (for hydrochloride salts). Pyridine bases in alcoholic samples containing resinous/volatile matter are estimated in the filtrate obtained after their treatment with alum at pH 5-6. The amount of pyridine and a-picoline is calculated from calibration graphs obtained by plotting the square of the spot diameter against pyridine and a-picoline concentrations. The method allowed detection and estimation of pyridine bases in denatured alcoholic samples at a concentration as low as 60 mg/L. Average recovery of added pyridine bases was 99.4% by this method.
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