Abstract
A simple paper chromatographic method for separation and quantitative determination of tetracycline in pharmaceutical preparations was developed. The method is based on the complexation of the antibiotic with a mixture of urea and disodium edetate on a chromatographic paper at pH 7.4. Spots of tetracycline and its degradation products were located under UV light, and their respective Rf values were calculated. The method can be used to determine tetracycline phosphate, even in the presence of oleandomycin. The developed method proved satisfactory in monitoring the stability of the antibiotic base and its hydrochloride.
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