Paper-Based Colorimetric Sensor for Hydrogen Peroxide Based Upon a Graphene Oxide/Platinum-Cobalt Nanocomposite

Abstract

The peroxidase-like properties of a graphene oxide/platinum-cobalt nanocomposite and the rapid color development mechanism of 3,3′,5,5′-tetramethylbenzidine (TMB) through the decomposition of ·OH from H2O2 were utilized to prepare a nonlabelled, simple, and sensitive paper-based colorimetric sensor. This sensor allows visualization of the results and instantaneous detection, enabling quantitative measurement of H2O2. The graphene oxide/platinum cobalt composite was synthesized using a two-step procedure. Its properties were investigated using transmission electron microscopy (TEM), Raman spectroscopy, and energy-dispersive X-ray spectroscopy (EDS). The composite was subsequently transferred onto a paper substrate to create the colorimetric sensor. The optimal catalytic conditions were a composite concentration of 201.17 µg/mL, a color development time of 3 min, a TMB concentration of 2 mmol·L−1, and a pH of 4. Using the optimal conditions, the paper-based colorimetric sensor has a linear range for H2O2 from 1.0 × 10−5 to 0.1 mol·L−1, with a limit of detection of 1.0 × 10−6 mol·L−1 which is comparable or better than comparable methods. This paper-based colorimetric sensor has potential applications for the rapid determination of hydrogen peroxide.