Paper-based array chip for multicolor visualized detection of shrimp allergen-specific IgE in serum

Abstract

Shrimp is a primary trigger of food allergies, and the assay for shrimp allergen-specific immunoglobulin E (sIgE) is a highly accurate and effective method for diagnosing shrimp allergies. In this study, we developed a paper-based array chip (PA-chip) capable of exhibiting multiple color changes for detecting sIgE to various Litopenaeus vannamei allergens (Lit v 1, Lit v 2, and Lit v 4). The PA-chip was utilized to measure sIgE levels in 12 non-allergic volunteers and 36 shrimp-allergic patients. The sensitivities for Lit v 1-sIgE, Lit v 2-sIgE, and Lit v 4-sIgE were 100.00%, 83.33%, and 91.67%, respectively, with specificities of 91.67%, 100.00%, and 91.67%, respectively. The agreement between this method and enzyme-linked immunosorbent assay results was 91.67%. Notably, the distinct colors on the PA-chip corresponded to varying levels of serum sIgE, offering predictive value for the severity of allergic reactions. In a word, the current study provides technical validation for allergen component-specific diagnosis.