PAPAYA SEED EXTRACT LOWERS SPERM CONCENTRATIONS, MOTILITY AND VIABILITY IN MALE MICE

Abstract. Alfarabi M, Siagian FE, Cing JM, Suryowati T, Turhadi, Suyono MS, Febriyamti MS, Naibaho FB. 2022. Bioactivity and metabolite profile of papaya (Carica papaya) seed extract. Biodiversitas 23: 4589-4600. Papaya (Carica papaya) seeds are part of the papaya plant which is a source of organic waste. However, there are many scientific studies thatstate the metabolites found in papaya seeds have various benefits in medical aspects. Accordingly, the objective of this study was to examine the antioxidant and cytotoxic activity of California and Bangkok type papaya seeds which are two local papaya types and widely consumed by the people of Indonesia. These papaya seeds'metabolite profiling was also conducted in this study therefore the antioxidant activity and cytotoxic mechanisms can be estimated. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) method is used to investigate the antioxidant activity while 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) isused to investigate the cytotoxic effect of extract. Metabolites contained in papaya seeds were measured by Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography-Mass Spectrometry-QTOF (LC-MS/MS-QTOF). The results showed that California and Bangkok papaya seed extracts had antioxidant activity with IC50being 24.4 ppm and 22.2 ppm, respectively. Both extracts were also able to inhibit the growth of cancer cells (MCF-7) and did not render any toxic effects on non-cancer cells at low concentrations. The metabolites contained in the two extracts were alkaloid, phenol hydrocarbon, flavonoid, fatty acid, and terpenoid groups. This study showed that papaya seeds have the potential to be further developed in medical field, especially as natural antioxidants and natural cancer-preventing ingredients.

So far men as a subject in family planning program had no priority, however recently men become a focus. Established mothodes for male contraception are through condom and vasectomy. Using condoms create psychological complaints, whereas vasectomy although very effective has often permanent effect. An other method of contraception is hormonal; besides that it is important to develop contraception using plants with antifertility effect such as papaya seed. Therefore, the aim of this research is to know the effect of extract papaya seeds on concentration and viability of sperms in vas deferens of male rat Strain LMR. This research was done using papaya seed extract, Bangka variety with 7 treatments, doses/kg/body weight, including 0 mg; 0.1 mg; 0.5 mg; 0.9 mg; 1.0 mg; 5.0 mg; 9.0 mg for times each treatment. Administration of papaya seed extract was performed by intramusculary injection for 20 days (1,5 seminiferous epithelium cycles). Investigation were done on 1) sperms concentration of vas deferens, 2) weight of testis, 3) seminiferous tubules diametric, 4) condition of spermatogenic cells. Injection with papaya seed extract for 20 days increased sperm concentration of vas deferens significantly (P<0,01), decreased population of spermatogonium A and primary spermatocytes preleptoten significantly (p<0,05), did not give any significant effect on weight of testis, seminiferous tubules diametric, primary spermatocytes pachyten and spermatid (P>0,05)

Activated carbons (ACs) were prepared from papaya seeds with different dry weight impregnation ratios of zinc chloride (ZnCl2) to papaya seeds by using a two-stage self-generated atmosphere method. The papaya seeds were first semi-carbonized in a muffle furnace at 300 °C for 1 h and then impregnated with ZnCl2 before activation at 500 °C for 2 h. Several physical and chemical characteristics such as moisture, ash, pH, functional groups, morphological structure and porosity of prepared ACs were studied and presented here. AC2, with the impregnation ration of 1: 2 (papaya seeds: ZnCl2), yielded a product that had the highest adsorption capacity, 91.75%, achieved after 180min contact time. The maximum Brunauer, Emmett and Teller (BET) surface area of AC2 was 546m2/g. Adsorption studies indicated that AC2 complied well with the Langmuir isotherm (qm=39.683mg g−1) and the pseudo-second-order (qe=29.36mg g−1). This indicated that chemisorption was the primary adsorption method for AC2. The intraparticle diffusion model proved that the mechanism of adsorption was separated into two stages: the instantaneous stage and the gradual adsorption stage. Overall, this work demonstrated the suitability of using papaya seeds as a precursor to manufacture activated carbon.

Endophytic bacteria were produce antibacterial compounds from various plants has been widely reported as considered efficient in overcoming resistant pathogen infections. This study aims to obtain endophytic bacteria from papaya seeds which can produce antibacterials. Papaya seeds were used to isolate endophytic bacteria, which were then examined for antibacterial activity. The dics diffusion method is used to determine the antibacterial activity's efficacy against Escherichia coli and Staphylococcus aureus. There are nine isolates of endophytic bacteria in all. According to a potency test, the nine isolates exhibit strong antibacterial activity against Escherichia coli and Staphylococcus aureus, respectively. Every isolate has the ability to effectively inhibit Staphylococcus aureus. The only isolates that could suppress Escherichia coli were CPA 5, CPA 6, and CPA 8. Based on their antibacterial activity against Escherichia coli and Staphylococcus aureus, it was determined that the endophytic bacterial isolates from papaya seed have the capacity to generate antibacterials.

Background and Aim:The brain is one of the most complex and crucial organs of our body. Its health is a matter of concern for all individuals as the number of aged people is increasing gradually in the world. Carica papaya is a ubiquitous plant, and its different parts possess neuroprotective effects against various neurodegenerative diseases. However, its brain anti-aging effects have remained uninvestigated. Therefore, this study has examined the brain anti-aging strength of C. papaya pulp and seeds extracts in D-galactose-induced aging rats.Methods:The rats were intraperitoneally injected with 150 mg/kg of D-galactose for 8 consecutive weeks to induce brain aging. In parallel, the rats of papaya pulp and papaya seed treated groups were injected with 150 mg/kg papaya pulp extract and 150 mg/kg papaya seed extract, respectively. The negative control group was only injected with 0.9% saline, whereas in the rats of the positive control group along with D-galactose 100 mg/kg VC was injected. After the treatment period, different neurobehavioral, neurochemical, and antioxidant analyses were performed to unmask the anti-aging strength of C. papaya pulp and seeds extracts.Results:C. papaya pulp and seed extracts significantly improved cognitive learning skills, memory, and muscular strength in aging rats while reducing stress and anxiety levels. Moreover, they enhanced neurotransmitters concentration and reduced oxidative stress. However, the anti-aging effects of C. papaya pulp were more significant than seeds.Conclusion:These results suggest that both C. papaya pulp and seed extracts possess neuroprotective effects against brain aging or age-related brain deteriorations but the age-protecting capability of C. papaya pulp is higher than C. papaya seeds. Therefore, it could be utilized as a component to design a novel brain anti-aging drug.Relevance for Patients:Brain aging is a natural process that every individual experiences in his life. The regular consumption of C. papaya can improve the quality of life by protecting neurons from age-related deteriorations.

Antimicrobial agents are abundant in medicinal plants. Many powerful and useful medications are derived from medicinal plants, which are used in various countries. Carica papaya roots, fruits, bark, seeds, and pulp have been shown to have therapeutic effects. It has been used to treat conditions such as eczema, warts, sinusitis, cutaneous tubercles, dyspepsia, blood pressure, amenorrhoea, and constipation. Carica papaya is a more important medicinal herb that is used as a folk medicine for the treatment of different disorders all over the world. Carica papaya seeds were approved and confirmed in some studies for their effective anthelmintic properties against nematodes found in animals. It is a member of the Caricaceae family. Carica papaya (Papaya) seeds were extracted using a soxhlet apparatus using solvents such as ethanol, methanol, and chloroform. The antibacterial and antifungal activity of extracts at various concentrations (50, 100, and 150 \(\mu\)g/ml) was examined using the agar disc diffusion method with three gramme positive, three gramme negative bacteria, and two fungal species. For antibacterial and antifungal activities, gentamicin and fluconazole were chosen as standard medicines. Methanol and chloroform extracts inhibited gramme negative germs more effectively than ethanol extract. Ethanol and chloroform extracts demonstrated a larger zone of inhibition (10-15 mm) than methanol extract for gramme positive microorganisms. Candida albicans exhibited a 15, 16 mm zone of inhibition in chloroform and methanol extracts at concentrations of 150 \(\mu\)g/ml, respectively. Similarly, Aspergillus niger exhibited a zone of inhibition of 11, 13 mm in 150 \(\mu\)g/ml methanol and chloroform extracts, respectively. According to the findings of these investigations, Carica papaya seed extracts have very strong antibacterial and antifungal activity in both methanol and chloroform extracts.

Papaya seeds (&lt;em&gt;Carica papaya &lt;/em&gt;L&lt;em&gt;.&lt;/em&gt;) and gandarusa leaves (&lt;em&gt;Justicia gendarussa &lt;/em&gt;Burm.f.) are considered medicinal plants that can be used as natural male contraceptives. Papaya seeds contain alkaloids, triterpenoids, saponins, and papain. Meanwhile, the gandarusa leaves contain flavonoids. These compounds have the function of antifertility for men. The purpose of this study was to determine the effect of papaya seeds (&lt;em&gt;Carica papaya &lt;/em&gt;L&lt;em&gt;.&lt;/em&gt;) ethanol extracts, gandarusa leaves (&lt;em&gt;Justicia gendarussa &lt;/em&gt;Burm.f&lt;em&gt;.&lt;/em&gt;), and its combination on motility, viability, and morphology of male mice spermatozoa (&lt;em&gt;Mus musculus&lt;/em&gt;&lt;em&gt;)&lt;/em&gt; most effective decrease concentration on motility, viability, and morphology of male mice spermatozoa (&lt;em&gt;Mus musculus&lt;/em&gt;). This research was an experimental study. The samples of this study were 18 male mice with a body weight of 20-30 grams, aged 3-5 months, and were divided into six groups. The treatments given were control, papaya seed ethanol extract, gandarusa leaves ethanol extract, and its combinations to concentrations of P1 Na CMC1%, P2 (papaya seed 100mg/kgW), P3 (gandarusa leaf 50mg/kgW), P4 (papaya seed : gandarusa leaf 50:50 mg/kgW), P5 (papaya seed : gandarusa leaf 50:100 mg/ kgW), P6 (papaya seed : gandarusa leaf 100:50 mg/kgW). The extract was given for 35 days. On the 36&lt;sup&gt;th&lt;/sup&gt; day, the mice were dissected to take their epididymis to observe the spermatozoa's quality (motility, viability, morphology). Those observations used a light microscope. Data were analyzed using one-way ANOVA and LSD post hoc tests. This study resulted differences in P1, P2, P3, P4, P5, and P6 towards motility, viability, and morphology of spermatozoa (&lt;em&gt;p&lt;/em&gt;&amp;lt; 0.05). The conclusion of this study showed that both single and combination ethanol extracts of papaya seeds and gandarusa leaves could reduce the motility, viability, and morphology of spermatozoa of male mice, and the effective dosage in reducing motility, viability, and morphology of spermatozoa is P3 gandarusa leaves 50mg/kgW.

Industrial effluent contaminants include insoluble substances, heavy metals and organic and inorganic compounds. The textile industry has a hard time dealing with its waste because it contains a lot of different colours and chemicals. To treat industrial effluent, coagulation-flocculation is frequently employed due to its efficacy in removing suspended particles organic matter, turbidity and colour. Conversely, employing a chemical coagulant may result in significant costs and the production of considerable quantities of non-biodegradable waste and metallic byproducts in the treated water, both of which face contamination risks by pollutants and diseases. An environmentally sustainable, non-toxic and biodegradable alternative method under consideration of utilising natural coagulants derived from plants, namely papaya seed and date seed. This study uses different NaCl concentrations to see how well papaya seed and date seed work as natural coagulants in treating textile industry wastewater. NaCl was the solvent and distilled water was used to extract the natural coagulant. Chemical Oxygen Demand (COD), turbidity, ammonia nitrogen and pH were assessed to evaluate the textile wastewater sample's response to papaya and date seed purification. The experiment's findings indicate that papaya and date seeds possess exceptional coagulation properties. The optimum turbidity reduction efficiency for papaya seed was 61.48% when 30 ml of a 1.0 NaCl concentration solvent was utilised. On the other hand, date seed removed turbidity with an efficiency of 83.96% when 10 ml of a 1.0 NaCl solvent was utilised. Aside from that, 1.0M NaCl was found to be the most effective COD reduction solution for the Carica papaya seed, while 2.0M NaCl resulted in a 56.19% reduction for the date seed. In addition, 30 ml Carica papaya seed coagulant dosage containing 2.0M NaCl produced the most significant reduction in ammonia nitrogen (82.84%). Also, the dosage of 20 ml date seed coagulant containing 2.0M NaCl eliminated 43.33% of ammonia nitrogen. The study's findings illustrated the potential advantages of utilising papaya and date seeds as organic coagulants in the remediation of effluents from the textile industry.

Papaya seed (Carica papaya L.) is known to be used as an herbal contraceptive. Papaya seeds contain anti-fertility compounds that can suppress fertility by interfering the ovarian function. The aim of this research was to find out the antifertility effect of papaya seed extract (Carica papaya L.) to the number and diameter of the Graafian follicle in female mice (Mus musculus). This research was conducted using Complete Randomized Design (CRD). Laboratory animals were treated with K(-) (Control with solvent); K(+) (Control with the contraceptive pill); P1, P2, and P3 (Treatment with doses of 1.4 mg, 3.5 mg, and 7 mg papaya seed, respectively, for each individual). The treatment was conducted for 21 days. The observed parameters were ovarian weight; body weight; number, diameter, and theca thickness of Graafian follicle. Data processing was performed using ANOVA test and tested further by Duncan test. All analyses were performed with a significance level of 5% (a = 0.05). Data were analyzed using SPSS version 16.0 computer program. The results showed that papaya seed extract was not significantly different (p> 0,05) on ovarium weight, body weight, Graafian follicle diameter, and theca layer thickness but significantly different (p <0,05) on the number of Graafian follicles in female mice. The conclusion of this research is the papaya seeds extracted by water can decrease the number of Graafian follicle so potentially to be used as an antifertility of female mice. Keywords : Antifertility, Papaya Seeds, Graafian follicle, Mus musculus

The present study was conducted to examine the ability of papaya (Carica papaya) seeds in reducing the reproductive performance of Nile tilapia through gonadal sterilization. In the first phase, 9 days fry of Nile tilapia were reared in 50-L size aquaria and fed with normal feed (T1) and papaya seed powder mixed feed @ 50 (T2), 100 (T3), and 150 (T4) g/kg diet at the rate of 5 percent of body weight for 30 days. After 30 days of treatment, 50 fish from each group were reared in outdoor hapa for 6 months with normal feed, and gonadal status was observed. In the second phase, matured fish from both the control and papaya seed treated group (T4@150 g/kg diet) were reared in two separate hapas for the next 3 months to observe their reproductive performance. Results showed that papaya seed feeding had no adverse effect on growth, survival, and water quality during the treatment period. The gonadosomatic index of both males and females was significantly lower in the papaya seed treated group (0.1±0.0 percent) than control (0.2±0.01 percent). The number of fish spawned per week was significantly lower in the papaya seed treated group (1.2±0.2) compared to the control group (3.6±0.3). The number of eggs per g female was significantly lower in the papaya seed treated group compared to the control group. The fertilization rate was significantly lower in the papaya seed treated group (93.6±1.1 percent) compared to the control group (98.2±1.3 percent) (P˂0.05). This study demonstrated that feeding papaya seeds at the dose of 150 g/kg diet (15 percent) can reduce the reproductive performance of Nile tilapia.

This decade, papaya seeds are known as waste rich in nutritional and nutraceuticals. However, their high moisture content can increase enzymatic and microbial activity, so drying them is critical. The effect of drying temperature (40 – 85°C) on the drying kinetics and effective moisture diffusivity coefficient (Deff) of papaya seeds under a hot-air oven dryer (HAOD) were investigated. In general, an increase in the drying temperature accelerated the drying process, indicated by a faster drying rate and shorter drying time. At high drying temperatures, the drying process was more dominant in the falling drying rate period than the constant drying rate period. Among the empirical and semi-theoretical thin-layer drying models studied, the logarithmic model is considered the most suitable model for predicting the moisture ratio of drying papaya seeds. Moreover, the Deff values of papaya seeds with and without sarcotesta ranged from 1.2 × 10−11 - 1.41 × 10−10 and 6.2 × 10−11 – 2.23 × 10−10 m2/s respectively, and they increased with the increasing drying temperature. Overall, HAOD shows good drying performance in kinetics and moisture diffusion, and can be applied for drying papaya seeds to safe moisture content. However, evaluations related to the energy consumption, exergy, and nutritional content of the dried papaya seeds need to be carried out in future studies.

Papaya (Carica papaya) seeds together with squash (Cucurbita moschata) seeds was pelleted to be used as dewormer, to determine its antihelmintic effects on growing chicken. They were randomly divided into four treatments and four replication with five birds per treatment was assigned using Randomized Complete Block Design (RCBD), with a total of eighty native chickens. The treatments were: Treatment A - Control (commercial dewormer) based on manufacturers recommendation; Treatment B - 75% Squash seeds and 25% Papaya seeds; Treatment C - 50% Squash seeds and 50% Papaya seeds; Treatment D - 25% Squash seeds and 75% Papaya seeds. The birds were treated orally at 2.5g of the pelleted dewormer based on the ratio of the study. In this study Treatment C, shows were highly influential in all parasites present in the chicken but of no significant result, also found to be at a lower cost.

The proximate and selected mineral composition of seeds and skin of ripe matured pawpaw (Carica papaya) were evaluated using standard methods. Ripe matured pawpaw fruits were washed, peeled, cut and the seeds and skin collected, air dried for 14 days at room temperature. The proximate composition determined using AOAC (2000) method revealed 11.02% and 10.22% moisture contents for both seeds and skins. Crude protein of 27.42% for seeds and 14.36% for skin were also obtained. Pawpaw seed contained 27.62% oil while the skin had 2.54% oil. Ash content of 5.22% (seeds), 11.03% (skin) were obtained respectively for the two samples. 8.02% of crude fibre was present in the seeds while 35.23% ash was present in the skin. The carbohydrate contents for both seeds and skin were 19.71% and 37.33% respectively. The selected minerals revealed 6.43mg/100g and 16.23mg/100g Calcium, 720.83mg/100g and 504.33mg/100g Potassium, 4.21mg/100g and 2.73mg/100g Iron while 6.42mg/100g and 1.94mg/100g Zinc were present in papaya seeds and skin respectively. However, it is evident that papaya seeds and skin, which are often discarded, contained essential nutrients that are useful for both humans and animal.

Carica papaya is known to reduce the quality of spermatozoa in morphology and viability of spermatozoa. Biochemical compound in Carica papaya is unstable and can easily degraded in disgetive track. Formulation of papaya seeds in the form of self-nano emulsion (SNE) is an alternative drug delivery system to increase bioavailability by lipophilic means. The sample consisted of 18 rats which were divided into 3 groups, namely the control group which was not given any treatment; the treatment group which was given papaya seed ethanol extract as much as 100 mg/kgBW and the treatment group which was given papaya seed extract-SNE as much as 100 mg/kgBW, the administration was carried out orally for 28 days. Examination of the quality of rat spermatozoa includes morphology, motility, and viability. Data analysis using One Way ANOVA and Chi-Square test. The results of the analysis showed that the decrease in morphology and viability of spermatozoa was significantly different with a result (p &lt; 0.05) between treatment groups. As for motility, it does not have a significant difference with a result (p &gt; 0.05) between treatment groups.. Based on the results of the study, it can be concluded that the administration of papaya seed ethanol extract and papaya seed SNE extract significantly reduces the quality of spermatozoa in the form of morphology and viability, and does not significantly reduce the motility of Wistar strain white rat spermatozoa.

Background: Men and women have the same rights and obligations in family planning program. Practically, participation in family planning program is still predominated by women while participation of men is still low. Low participation of men in family planning program is due to very limited choices in male contraceptive method. In recent years, studies have been refocused on investigating traditional plants as antifertility herbal medicine for men. Studies on antifertility effects of unripe papaya seeds have been done. However, similar studies on hexane fraction extract have not yet been done. In this study, unripe seeds were collected from local Balinese papaya ( Carica papaya ) fruits. Objective: This study was aimed to assess the effect of unripe papaya seeds hexane fraction extract on spermatogenesis and the testosterone level of male mice. Hexane fraction extract of unripe papaya seeds contains glycosides and triterpenoids, which is assumed to have an antifertility property, so it can be used as a male contraceptive, although the mechanism of action remains to be elucidated. Method: This study used pre-test and post-test control group design, using 30 male mice of balb C strain, 12 weeks of age, weighing 20-22 gram, which were randomly grouped into 3 groups, each consisting of 10 male mice. One control group (P0 = control group) was given double-distilled water, and two treatment groups were given hexane fraction extract of unripe Carica papaya seeds 20 mg/20gram/day, and methanolic extract of unripe Carica papaya seeds 20 mg/20 gram/day (P1 and P2, respectively). After 36 days of treatment, evaluation of the testes and blood of the male mice was conducted. Results: Data were analysed by normality test of Kolmogorov-Smirnov Goodness of Fit, homogeneity test, and Anova test. This study showed that spermatogonia A, primary pachytene spermatocytes, spermatid and Sertoli cells were decreased significantly (p 0,05). Conclusion: It can be concluded that hexane fraction extract of unripe Carica papaya seeds can decrease the mean number of spermatogonia A cells, spermatocyte of primary pachytene spermatocytes, spermatid and Sertoli cells better than methanolic extract of unripe Carica papaya seeds.