Papaya recombinant inbred lines selection by image-based phenotyping

Diego Fernando Marmolejo Cortes,Nádia Fernandes Moreira,Alexandre Pio Viana,Messias Gonzaga Pereira,Alinne Oliveira Nunes Azevedo,Helaine Christine Cancela Ramos,Geraldo Antônio Ferreguetti,Renato Santa-Catarina,Julio Cesar Fiorio Vettorazzi,Tathianne Pastana de Sousa Poltronieri

doi:10.1590/1678-992x-2016-0482

Diego Fernando Marmolejo Cortes, Nádia Fernandes Moreira + Show 8 more

Open Access

https://doi.org/10.1590/1678-992x-2016-0482

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Abstract

ABSTRACT: The selection of superior Carica papaya (L) genotypes depends on the availability of genetic variability and on the favorable and simultaneous response of the genotypes to those traits of most interest. However, manual phenotyping (MP) demands intensive labor, is time-consuming and expensive. The aim of the current study is to access the efficiency of image-based phenotyping (IBP) in estimating genetic parameters and in selecting F4 recombinant inbred lines. The genetic parameters and values were estimated in accordance with the REML/BLUB procedure and combined selection using the selection index based on standardized genetic values. The majority of traits accessed through IBP showed experimental coefficients of variation similar to those found through MP. Both methodologies showed genetic parameters of similar magnitude, indicating expressive genetic variability between lines in the traits accessed in this study. The same superior lines were indicated in both methodologies and expressive genetic gains obtained through the lines were selected for all traits. IBP performance was similar to that of MP with respect to the estimates of breeding-relevant traits such as commercial fruits and yield. Thus, IBP showed efficient phenotypic assessment, as well as selective accuracy in accessing genetic variability and genetic gains, when it was compared to MP. Since IBP is far less dependent on labor, it is expected to be incorporated into the routine of papaya breeding programs as a way of increasing the number of accessed lines and, consequently, increasing genetic gains.

Highlights

Papaya (Carica papaya L.) is one of the most economically important fruits in the tropical and subtropical regions of the world
The genetic parameters and values were estimated in accordance with the Restricted Maximum Likelihood (REML)/BLUB procedure and combined selection using the selection index based on standardized genetic values
The CVe values estimated for plant height (PH) and stem diameter (SD) were low indicating a high degree of experimental precision

Summary

Introduction

Papaya (Carica papaya L.) is one of the most economically important fruits in the tropical and subtropical regions of the world. It is widely known for its nutritional benefits and pharmacological properties (Oliveira and Vitória, 2011). The development of cultivars depends on the availability of genetic variability and the simultaneous and favorable response of the genotypes to most traits of agronomic importance. The phenotypic assessment of fruit tree genotypes requires intensive labor and is based mainly on traits such as fruit yield and quality. Phenotypic assessment affects the selection strategies and it is necessary to the development of methodologies which will efficiently collect, store and analyze data (Merk et al, 2012)

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