Abstract
Magnetic nanocrystalline cellulose (MNCC), a novel biobased nanocomposite, was prepared via a simple coprecipitation-cross-linking technique and structurally characterized. Papain (PA) was successfully immobilized onto the MNCC. The resulting nanobiocatalyst PA@MNCC showed high PA loading (333 mg/g) and enzyme activity recovery (more than 80%). The stability of PA@MNCC was greatly superior to that of its free counterpart. Also, PA@MNCC manifested markedly enhanced solvent tolerance. The secondary structure study of the enzyme proved that these enhancements were attributed to the increase of structure rigidity of PA@MNCC. The observed optimum pH and temperature of PA@MNCC were significantly higher than the corresponding levels of free PA. A kinetic study demonstrated that PA@MNCC had an increase in enzyme-substrate affinity. Furthermore, the as-prepared PA@MNCC was successfully used as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-glutamine (Z-Ala-Gln) dipeptide in deep eutecti...
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