Abstract
BackgroundPannexins (Panxs) are relatively newly discovered large-pore ion and metabolite permeable channels. Although no proteomics-based interactome has yet been published, Panx1 has been demonstrated to interact with actin in an ectopic expression system. This interaction affects both Panx1 plasma membrane stability as well as cytoskeletal remodelling. The current study builds on our recent discovery of Panx1 expression in ventricular zone (VZ) neural stem and progenitor cells (NSC/NPCs), and on the demonstrated interaction of Panx1 with the cytoskeleton.FindingsHere we demonstrate that Panx1 also plays roles in two additional cell behaviours associated with neurogenesis, including cell migration and neurite extension. Furthermore, we confirm an endogenous interaction between actin and Panx1, and identify a new interaction with actin-related protein 3, an actin cytoskeleton-modulating protein.ConclusionsThis study further establishes the importance of Panx1 in the cell biology of NSC/NPCs and strengthens and expands our knowledge of Panx1 interactions with the cytoskeleton.
Highlights
Pannexins (Panxs) are relatively newly discovered large-pore ion and metabolite permeable channels
Panxs are four-pass transmembrane proteins that oligomerize to form large-pore mechanosensitive channels permeable to ions and metabolites of up to 1 kDa in size, such as adenosine triphosphate (ATP) [1,2]
We recently detected Panx1 expression in the Neuro-2a (N2a) cell line as well as in post-natal ventricular zone (VZ) neural stem and progenitor cells (NSC/NPCs), where it positively regulates cell proliferation in part through release of ATP that results in activation of purinergic receptors
Summary
Pannexins (Panxs) are relatively newly discovered large-pore ion and metabolite permeable channels. Conclusions: This study further establishes the importance of Panx1 in the cell biology of NSC/NPCs and strengthens and expands our knowledge of Panx1 interactions with the cytoskeleton. A recent study in glioma cells further supported a role for Panx1 in the dynamic regulation of actin cytoskeleton remodeling [7].
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