Abstract
A novel strain LTYR-11ZT that exhibited multiple plant growth promoting (PGP) traits was isolated from the surface-sterilized leaves of Alhagi sparsifolia Shap. (Leguminosae), which reprsents one of the top drought tolerant plants in north-west China. Phylogenetic analysis of 16S rRNA gene sequences and multilocus sequence analysis based on partial sequences of atpD, gyrB, infB and rpoB genes revealed that strain LTYR-11ZT was a member of the genus Pantoea, with Pantoea theicola NBRC 110557T and Pantoea intestinalis DSM 28113T as the closest phylogenetic relatives. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analysis confirmed that strain LTYR-11ZT represents a novel species of the genus Pantoea, for which we propose the name Pantoea alhagi sp. nov. Confocal microscopy observation revealed that strain LTYR-11ZT effectively colonizes the rhizoplane of both Arabidopsis and wheat. Strain LTYR-11ZT was able to promote the growth of wheat enhancing its resistance to drought stress. Strain LTYR-11ZT led to increased accumulation of soluble sugars, decreased accumulation of proline and malondialdehyde (MDA), and decreased degradation of chlorophyll in leaves of drought-stressed wheat. Our findings will contribute to the development of a novel biotechnological agent to improve the adaptation of crop plants to drought in arid ecosystems.
Highlights
Conditions[13,14,15]
In both neighbour-joining (NJ) and maximum-likelihood (ML) phylogenetic trees based on 16S rRNA gene sequences (Fig. 1, Fig. S1), Pantoea and Erwinia species were shown to be polyphyletic, which is in agreement with previous reports[20,21]
Strain LTYR-11ZT was found to form a coherent cluster with P. theicola NBRC 110557T and P. intestinalis DSM 28113T, suggesting that strain LTYR-11ZT belongs to the genus Pantoea
Summary
Conditions[13,14,15]. it is a good strategy to look for plant-beneficial microorganisms that confer resistance to a specific environmental stress from the soil environments where that stress is a regular phenomenon[3]. (Leguminosae), a typical desert plant species, is widely distributed in the arid and salinized regions of north-west China. This perennial plant has highly developed deep roots and possesses a great capacity to withstand poor soil and severe drought conditions, thereby playing a fundamental role in maintaining the desert ecosystem[16]. A. sparsifolia may represent an ideal species for discovering novel biotechnological agents for use in arid land agriculture On this background, A. sparsifolia was collected from the Taklamakan Desert, Xinjiang Uyghur Autonomous Region, north-west China, and endophytic bacterial communities from its leaves, stems and roots were isolated and subjected to in vitro tests for PGP traits and abiotic stress tolerance. LTYR-11ZT will be helpful for the further investigations into the mechanisms behind bacteria-mediated drought tolerance in plants
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