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Abstract
BackgroundRice blast is the most serious disease afflicting rice and there is an urgent need for the use of disease resistance (R) genes in blast tolerance breeding programs. Pb1 is classified as a quantitative resistance gene and it does not have fungal specificity. Pb1-mediated resistance develops in the latter stages of growth. However, some cultivars, such as Kanto209 (K209), cultivar name Satojiman, despite possessing Pb1, do not exert resistance to rice blast during the reproductive stage.ResultsWe found that the expression of WRKY45 gene downstream of Pb1 was weakly induced by rice blast inoculation at the full heading stage in K209. Genetic analysis using the SNP-based Golden Gate assay of K209 crossing with Koshihikari Aichi SBL (KASBL) found at least four regions related to the resistance in the rice genome (Chr8, Chr9, Chr7, Chr11). Mapping of QTL related to Chr7 confirmed the existence of factors that were required for the resistance of Pb1 in the 22 to 23 Mbp region of the rice genome.ConclusionWe clarified how the K209 cultivar is vulnerable to the blast disease despite possessing Pb1 and found the DNA marker responsible for the quantitative resistance of Pb1. We identified the QTL loci required for Pb1-mediated resistance to rice panicle blast. Pb1 was negatively dependent on at least three QTLs, 7, 9 and 11, and positively dependent on one, QTL 8, in the K209 genome. This finding paves the way for creating a line to select optimal QTLs in order to make use of Pb1-mediated resistance more effectively.
Highlights
Rice blast is the most serious disease afflicting rice and there is an urgent need for the use of disease resistance (R) genes in blast tolerance breeding programs
We evaluated the index of panicle blast severity of the cultivars at the Aichi Agricultural Research Center, Mountainous Resin Agricultural Research Institute
Genetic analysis of Koshihikari Aichi SBL (KASBL) and K209 revealed that the regions Chr8, Chr9, Chr7, and Chr11 were involved in rice blast resistance in at least four rice genomes (Fig. 4)
Summary
Rice blast is the most serious disease afflicting rice and there is an urgent need for the use of disease resistance (R) genes in blast tolerance breeding programs. Pb1-mediated resistance develops in the latter stages of growth. Some cultivars, such as Kanto209 (K209), cultivar name Satojiman, despite possessing Pb1, do not exert resistance to rice blast during the reproductive stage. In order to minimize the damage of rice blast, resistance (R)-gene cultivars are commonly used. The use of R genes to develop resistant cultivars has become an urgent goal in rice breeding programs. Several molecular approaches to study rice blast resistance genes have been developed during the last decade. Hundreds of blast R genes have been mapped in the rice genome.
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