Abstract
The insulin, glucagon, somatostatin and pancreatic polypeptide content of acid-ethanol extracts of pancreas from lean and obese (ob/ob) mice of various ages was determined by radioimmunoassay. Rat and mouse pancreatic polypeptide react weakly with antibodies to avian, bovine and canine pancreatic polypeptide, but immunoassay for this peptide was possible using an antibody to the carboxyl-terminal hexapeptide of bovine pancreatic polypeptide and amino-terminal labelled bovine pancreatic polypeptide as tracer. The insulin, glucagon and pancreatic polypeptide content of pancreas from obese mice was greater than that of lean controls. The increase in insulin content uniformly involved ventral, dorsal and splenic lobes. Glucagon content was elevated primarily in the splenic lobe. Pancreatic polypeptide content was most significantly elevated in the splenic lobe where pancreatic polypeptide cells are infrequent in normal lean mice and this was accompanied by increased numbers of pancreatic polypeptide cells per islet in this lobe.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
