Abstract
Purpose: We have shown in rats that vagotomy (V), celiac ganglionectomy (CG), V plus CG, and peri-Vaterian section and reanastomosis of the duodenal wall above and below the Vaterian papilla (PV-SRA) were associated with a sig. fall of lipase output in basal bile-pancreatic secretion (BPS). In contrast, Häkanson vagal reflex (2-hr pyloric ligation), that triggers an enhancement of the intrapancreatic cholinergic tone, reveals a non-significant modification of amylase but a marked sig. increase of lipasemia values. The aim of the present tests was to evaluate, taking into account our findings with the secretin test in menopause women of a sig. fall of lipase secretion in respect to those observed in men of the same age (>45 years), the effects of estradiol administration in male rats in comparison with a control solvent-treated series. Methods: Experiments were performed in male Wistar rats, bw˜300g. The experimental series was subjected to a subcutaneous injection of 50 μg of estradiol benzoate in 0.1 ml of peanut oil solvent. Estrogen was administered every other day for 15 days. In a control group of 13 animals, only the sucutaneous estrogen solvent was given for the same period. A biliary pancreatic fistula was created. BPS was collected for 180 minutes, in 30 min samples. Volume, bicarbonate concentration and output, both protein and lipase concentration and output were determined. Results: The 180 min cumulative data comparing estrogen-treated and control rodents disclosed no differences in the pattern of secretion (rate of flow and bicarbonate). Basal ecbolic secretion was augmented for protein and lipase concentration and output but only the latter revealed a markedly statistical significant increase. The present results, that of a selective rise in protein but primarily of lipase secretion (>200%) in the estrogen-treated rats, support the speculation that estradiol, in a potentiated interaction with the intrapancreatic cholinergic tone, constitute the main pivotal physiologic mechanism. The histologic finding in the estradiol-treated rats of “pancreon” acinar cells relatively clear and vitreous, without the classical granular appearance, suggestively reminds of what is seen after a pilocarpine injection. Conclusion: The present results, coherent with preceding ones, provide solid basis to our speculation that, indeed, estradiol effects on “pancreon” units are exerted in a close interplay with the level degree of an intrapancreatic cholinergic tone. Am J Gastroenterol 2009; 104:S82-S102; doi:10.1038/ajg.2009.492
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