Panax Notoginseng Saponins Restrains Ischemiareperfusion-induced Rat Mesenteric Microcirculatory Disturbance

Abstract

Objective: To investigate the effect of Panax notoginseng saponins (PNS) on ischemia reperfusion (I/R) induced rat mesenteric microcirculatory dysfunctions. Methods: Male Wistar rats weighting 200–250 g were subjected to 10 min ligation of the superior mesenteric artery and vein, followed by 60 min reperfusion. PNS (5 mg/kg/hr) was continuously administrated starting from 10 min before ischemia or 10 min after reperfusion until 60 min after reperfusion via left jugular vein. Leukocytes adhesion, mast cell degranulation, endothelial peroxidation, and albumin leakage of rat mesenteric venules were observed. Serum myeloperoxidase (MPO) level, intercellular adhesion molecule-1 (ICAM-1) expression and Src phosphorylation were examined. Results: PNS ameliorated leukocyte adhesion and mast cell degranulation, while with no obvious effects on endothelial peroxidation and albumin leakage. In addition, PNS inhibited serum MPO increase, intestinal ICAM-1 expression and Src phosphorylation induced by I/R. Conclusions: PNS ameliorated I/R-induced leukocyte adhesion and mast cell degranulation, the former is related to its inhibition of Src phosphorylation and ICAM-1 expression. Abbreviations: DHR, Dihydrorhodamine-123; FITC, Fluorescein isothiocyanate; ICAM-1, intercellular adhesion molecule-1; I/R, ischemia-reperfusion; MPO, myeloperoxidase; NF-κB, nuclear factor-kappa B; PN, Panax notoginseng; PNS, Panax notoginseng saponins; p-Src, phosphor-Src; TNF-α, tumor necrosis factor.