Abstract
Escherichia albertii is characterized as an emerging pathogen, causing enteric infections. It is responsible for high mortality rate, especially in children, elderly, and immunocompromised people. To the best of our knowledge, no vaccine exists to curb this pathogen. Therefore, in current study, we aimed to identify potential vaccine candidates and design chimeric vaccine models against Escherichia albertii from the analysis of publicly available data of 95 strains, using a reverse vaccinology approach. Outer-membrane proteins (n = 4) were identified from core genome as vaccine candidates. Eventually, outer membrane Fimbrial usher (FimD) protein was selected as a promiscuous vaccine candidate and utilized to construct a potential vaccine model. It resulted in three epitopes, leading to the design of twelve vaccine constructs. Amongst these, V6 construct was found to be highly immunogenic, non-toxic, non-allergenic, antigenic, and most stable. This was utilized for molecular docking and simulation studies against six HLA and two TLR complexes. This construct can therefore be used for pan-therapy against different strains of E. albertii and needs to be tested in vitro and in vivo.
Highlights
Escherichia albertii is an emerging gram-negative, mucocutaneous, non-motile, monophyletic bacterium, belonging to the Enterobacteriaceae family [1]
We applied the subtractive pan-genome analysis, followed by a reverse vaccinology approach on 95 strains, to identify vaccine candidates and design a novel vaccine construct against E. albertii
A pan-genomic analysis based reverse vaccinology approach was utilized to assess the novel potential vaccine candidate and design multi-epitope vaccine construct against E. albertii
Summary
Escherichia albertii is an emerging gram-negative, mucocutaneous, non-motile, monophyletic bacterium, belonging to the Enterobacteriaceae family [1]. It is the causative agent of foodborne illness and diarrhea, mostly in young children [2]. E. albertii was diagnosed in a 9-month-old diarrheic child in Bangladesh and identified through biochemical tests, as Hafnia alvei [3]. It was later named as E. albertii after M. E. albertii has various unique or noteworthy genetic traits, including those responsible for known biochemical properties and virulence factors, as well as an active T3SS [6]
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