Abstract

Palynological investigation on five cultivars ofChrysanthemun morifoliumRamat. and their induced somatic flower color mutants obtained through gamma irradiation and colchicine treatment has been carried out using LM and SEM. The objective of the work was to analyze the changes in the pollen morphology which occurred in mutagen-induced somatic flower color mutants and to verify if there is any correlation between the changes in flower color/type and pollen morphology. Chromosomal status of all the cultivars and their somatic mutants is hexaploid (6n= 54), but different types of chromosomal aberrations have been observed. Significant increase in pollen grain sterility was found in all the mutants. The pollen grains of all the cultivars and their mutants are basically 3(-4) zonocolporate with tectate spinose exine having perforations. No appreciable variation in pollen apertural character was noticed in any of the mutants. Significant changes in pollen exine surface pattern were found in 4 mutant varieties. The changes are inconsistent and do not correspond to the intensity of radiation.

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