Abstract
To investigate the effects of IVF aspiration on the temperature, pH, and dissolved oxygen of bovine follicular fluid. The temperature was monitored at various positions in an aspiration kit. Dissolved oxygen and pH were measured before and after aspiration. The temperature of follicular fluid dropped by 7.7 +/- 1.3 degrees C upon aspiration. Dissolved oxygen levels rose by 5 +/- 2 vol.%. The pH increased by 0.04 +/- 0.01. The temperature change was attributed mainly to evaporation of fluid in the collection tube. Changes in dissolved oxygen levels and pH were due to contact with air. Standard vacuum-based aspiration may induce changes in follicular fluid, which could be detrimental to oocyte health and affect attempts to correlate chemical characteristics with oocyte quality.
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