Abstract
PAIA assays: A new bead-based assay system for high throughput protein quantification
Highlights
The patent pending PAIA technology provides a novel platform for the quantification of proteins, e.g. monoclonal antibodies
PAIA assays consist of functionalized beads to capture the protein of interest and a fluorescent marker to detect the analyte
In contrast to other bead-based assays, which measure the fluorescence intensity of marker that is bound to the beads [1], PAIA assays measure the fluorescence of unbound marker remaining in solution
Summary
PAIA assays consist of functionalized beads to capture the protein of interest (the analyte) and a fluorescent marker to detect the analyte. We use protrusions on the bottom of the microplate that separate captured analyte-marker complexes from unbound fluorescent marker. No washing steps are needed and the assay can be performed in an automation-friendly 384-well plate format (Figure 1A).The time to result, the amount of sample required, and the hands-on time are substantially reduced compared to other immunoassay formats. This novel approach is suitable for high throughput analysis of protein containing samples like supernatants of antibody producing cells
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
