Abstract
Perfusion of noncytotoxic concentrations of hydrogen peroxide (H 2O 2) through canine carotid arteries potentiates neutrophil adhesion to vessel endothelium. Platelet-activating factor (PAF) receptor antagonists block neutrophil adhesion to vessels pretreated with low millimolar concentrations of H 2O 2. We have used a specific gas chromatographic-mass spectrometric (GC-MS) assay for PAF and applied this to studies of canine carotid arteries perfused with H 2O 2. Vessels perfused with 1 and 10 mM H 2O 2 for 20 min produced PAF in a dose-dependent manner, 331 ± 67 pg/g tissue with 1 mM H 2O 2 and 1160 ± 194 pg/g with 10 mM. Vessels that had been denuded of endothelium with a balloon catheter prior to H 2O 2 perfusion produced similar quantities of PAF in response to H 2O 2 (220 ± 72 pg/g and 960 ± 210 pg/g with 1 and 10 mM, respectively). Cultured canine jugular venous endothelial cells produced PAF in response to 10 mM H 2O 2 809 ± 117 pg 10 7 cells ) but carotid arterial smooth muscle cells did not. These results suggest that vascular cells other than endothelial cells may produce PAF following H 2O 2 perfusion of canine carotid arteries.
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