Paenibacillus quercus sp. nov., isolated from rhizosphere of Quercus aliena var. acuteserrata

Abstract

A Gram-positive, spore-forming, rod-shaped and motile bacterium, designated strain 1-25(T), was isolated from the rhizosphere of Quercus aliena var. acuteserrata in Taibai Mountain, Shaanxi Province, China. 16S rRNA gene sequence analysis showed that strain 1-25(T) belongs to the genus Paenibacillus. Strain 1-25(T) was found to be closely related to Paenibacillus harenae and Paenibacillus castaneae with 96.0 and 95.9% 16S rRNA gene sequence similarities, respectively. The strain was observed to grow optimally at 28°C and pH 7.5. The major isoprenoid quinone was found to be menaquinone-7. The dominant cellular fatty acids were identified as anteiso-C15:0 and iso-C15:0. The diagnostic diamino acid in the cell-wall peptidoglycan was found to be meso-diaminopimelic acid. The DNA G+C content was determined to be 41.6mol%. On the basis of phenotypic characteristics and molecular properties, strain 1-25(T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus quercus sp. nov. is proposed. The type strain is 1-25(T) (=CCTCC AB2013265(T)=KCTC 33194(T)).