Paenibacillus mucilaginosus TKU032生產生物界面活性劑與胞外多醣之條件與特性分析

Abstract

This study is based on an initial option of screening of biosurfactant-producing bacteria,after we choose thebacteria which produce biosurfactant,we found the strain of P. mucilaginosus TKU032 have some gell-like substance in the supernatant,and the color of supernatant will become deeper gradually.Preliminary experiments found that this gum is a polysaccharide.This study will be into two part:P. mucilaginosus TKU032 producing bio-surfactants and study of extracellular polysaccharides. With squid pen powder (SPP), head of the prawn shell powder (SHP) as a carbon / nitrogen source, according to different proportion (0.5% -2%) of the added concentration, at 25 ℃, 30 ℃, 37 ℃ cultured 0-5 days found to be 2% squid cartilage, 37 ℃ fermentation supernatant of cultured for 3 days to get the lowest surface tension. The same conditions can be obtained up to four days of training extracellular polysaccharide, to observe the fifth day of fermentation supernatant color black lead difficult to measure, and therefore a better choice of culture conditions as the above conditions are cultured P. mucilaginosus TKU032. TKU032 fermentation resulting supernatant, the surface tension of about 36.3 mN / m after NaOH to adjust pH values through 12, placed at 4 ℃, 24 hours, wait until the precipitate and the precipitate removed by centrifugation, the supernatant lyophilized and then eluted with methanol-soluble substance was concentrated under reduced pressure to dryness to obtain tan oil which is purified of the bio-surfactant. TKU032 fermentation supernatant obtained after heating (121 ℃, 20min) after bleaching crude extracellular polysaccharide (14.8g / L), then the use of crude extracellular polysaccharide Sevag reagent to protein, enzymatic hydrolysis, dialysis, it can be oligosaccharide structure into smaller substances; another access three different acid hydrolysis extracellular polysaccharide can be obtained in the structure of the carbohydrate is small, the use of nuclear magnetic resonance (NMR) spectra and matrix-assisted laser desorption ionization time of flight mass Instrument (MALDI-TOF) analysis after hydrolysis of oligosaccharide structures. In addition to fermentation squid pen as carbon / nitrogen source, in the first day of TKU032, the culture supernatant have higher DPPH scavenging ability (80%), and preferably the total phenolic content, reducing power. 表單編號 ：ATRX-Q03-001-FM0030-01