Paenibacillus glycinis sp. nov., an Endophytic Bacterium Isolated from the Nodules of Soybean (Glycine max (L.) Merr).

Abstract

A Gram-negative-staining, endospore-forming, rod-shaped bacterium, designated T1T, was isolated from root nodules of soybean (Glycine max (L.) Merr) in Heilongjiang Province of China. The isolate was identified as a member of the genus Paenibacillus based on phenotypic and phylogenetic characteristics. The 16S rRNA sequence was closely related to that of Paenibacillus sacheonensis SY01T with a similarity of 98.4%. Average nucleotide identity and in silico DNA-DNA hybridization values between strain T1T and P. sacheonensis DSM 23054T were 81.4% and 25.4%, respectively. The DNA G + C content of strain T1T was 58.2mol%. meso-Diaminopimelic acid was detected in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and iso-C15:0. The predominant respiratory quinone was MK-7. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five unidentified phospholipids, four unidentified aminophospholipids, an unidentified glycolipid and an unidentified lipid. Based on these results, T1T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus glycinis sp. nov. is proposed. The type strain is T1T (= CGMCC 1.18563 = KCTC43227).