Paenibacillus arenilitoris sp. nov., isolated from seashore sand and genome mining revealed the biosynthesis potential as antibiotic producer.

Abstract

Strain IB182493T, a marine, aerobic, Gram-stain-negative and motile bacterium, was isolated from seashore sand of South China Sea. Cells grew optimally at 25-30°C, pH 7.0-8.0 and with 2-4% NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Paenibacillus, and was most closely related to Paenibacillus harenae DSM 16969T (similarity 96.6%) and Paenibacillus alkaliterrae DSM 17040T (similarity 96.1%). The chemotaxonomic characteristics of strain IB182493T included MK-7 as the predominant isoprenoid quinone, anteiso-C15:0 and iso-C16:0 as the major cellular fatty acids and meso-diaminopimelic acid as the diagnostic diaminoacid in cell wall peptidoglycan. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. The DNA G + C content of strain IB182493T was 56.2%. The values of whole genome average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) between the isolate and the closely related type strains were less than 84.7% and 23.6%, respectively. On the basis of phenotypic and chemotaxonomic properties, phylogenetic distinctiveness and genomic data, we named thestrain as Paenibacillus arenilitoris sp. nov. and proposed that strain IB182493T (= MCCC 1K04626T = JCM 34215T) in the genus Paenibacillus represents a novel species.