Paenibacillus albidus sp. nov., isolated from grassland soil.

Abstract

A novel bacterial strain, designed Q4-3T, was isolated from a soil sample obtained from Qilian grassland, Qinghai, China. Phylogenetic, phenotypic, chemotaxonomic and molecular analyses were performed on the new isolate. Cells were Gram-stain-positive, facultatively anaerobic, spore-forming, motile rods with peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences placed strain Q4-3T in the genus Paenibacillus, and its closest relatives were Paenibacillus odorifer JCM 21743T, Paenibacillus typhae DSM 25190T, Paenibacillus borealis DSM 13188T and Paenibacillus etheri DSM 29760T with 16S rRNA gene sequence similarities of 98.12, 97.89, 97.63 and 97.6 %, respectively. The isolate grew at 4-37 °C (optimum 28-30 °C), at pH 6.0-10.0 (optimum pH 7.5) and with 0-3 %(w/v) NaCl (optimum 1 %). The DNA of strain Q4-3T was determined to be 48.6 mol%. The predominant menaquinone was MK-7 and the diamino acid in the cell-wall peptidoglycan was found to be meso-diaminopimelic acid. Anteiso-C15 : 0 (55.5 %), iso-C16 : 0 (14.5 %) and C16 : 0 (13.3 %) were the major fatty acids. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified aminophospholipids and one unidentified lipid. Based on these results, strain Q4-3T is considered to represent a novel of the genus Paenibacillus, for which the name Paenibacillusalbidus nov. is proposed. The type strain is Q4-3T (=CGMCC 1.16134T=KCTC 33911T).