Abstract
To develop an isolated working heart model with parabiotic circulation in swine and verify its stability and possibility to allow effective measurements of hemodynamic and metabolic data. This model was developed during study of association of agents to cardiology. There were performed 18 experiments, each with a support animal and a donor animal. Donor animal heart was perfused as isolated working heart with parabiotic circulation from support animal. Isolated heart underwent regional ischemia by interventricular artery clamping, followed by global ischemia. During reperfusion in working heart state mode at 30, 60 and 90 minutes, contractility indices such as elastance, preload recruitable stroke work index and metabolic data were acquired. Support animals were kept stable throughout the procedures without use of blood transfusions or vasoactive drugs. pH, oxygen partial pressure and hematocrit were kept stable and within physiologic ranges. Isolated heart was perfused adequately throughout the experiment. All hemodynamic and metabolic data proposed were adequately measured in working heart state mode. This swine isolated "working heart" model was kept stable throughout the experiments with no administration of vasoactive drugs and it allowed adequate measurements of metabolic and hemodynamic data.
Highlights
Since Langendorff the isolated heart models have been used successfully for the study of hearts in mammals [1]
This isolated swine “working heart” model was kept stable throughout the experiments with no administration of vasoactive drugs, and it allowed adequate measurements of metabolic and hemodynamic data
The coronary flow presented great dispersion in the results presented in St
Summary
Since Langendorff the isolated heart models have been used successfully for the study of hearts in mammals [1]. The primary isolated heart models were advanced with the use of parabiotic circulation by Heymans and Kochmann [3]. In such a model, a support animal is used to keep the isolated organ perfused and, despite the fact that these heart models require more complex preparations than the preparations with solutions crystalloids, they allow the model to have a greater closeness to the physiological situation [4]. The pig’s heart presents anatomical and physiological characteristics that allow its successful use as a model for comparison with the human heart [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
