Abstract
OBJETIVO: Padronizar um modelo experimental de lesão de medula espinal em ratos Wistar, utilizaram-se um equipamento computadorizado para impacto por queda de peso e os parâmetros determinados pelo Multicenter Animal Spinal Cord Injury Study - MASCIS. MÉTODOS: Avaliaram-se 30 ratos, com idade variando entre 20 e 25 semanas de vida. O peso variou de 200 a 300 g, para as fêmeas, e de 232 a 430 g para os machos. Realizaram-se impactos com pesos de 10 g de 12,5; 25 e 50 mm de altura, controlando-se a velocidade de impacto e o coeficiente de compressão. O impacto ocorreu sobre a superfície da medula espinal na altura da décima vértebra torácica, após laminectomia. Monitoraram-se os sinais vitais e realizaram-se gasometrias previamente e posteriormente à lesão da medula. O volume de lesão foi avaliado pela análise quantitativa dos íons de sódio e potássio. RESULTADOS: Verificaram-se correlações estatisticamente significantes entre o volume de lesão e os parâmetros mecânicos. O volume de lesão provocado por queda de 50 mm de altura foi superior aos de 12,5 e 25 mm, que não diferiram entre si. CONCLUSÃO: O modelo demonstrou-se eficaz e capaz de gerar lesões medulares padronizadas em ratos Wistar.
Highlights
The potentially devastating effect of spinal cord injuries on the patient’s quality of life is a cause for concern among physicians that care for this kind of patient
There was no statistical significance: in the distribution of frequency of gender, descriptive of age, of weight (g), of pH, of partial pressure of pO2, of pCO2, of O2 saturation (%) and of mean blood pressure of the rats prior to the contusion according to the height of the fall of the impact rod used by group
Our choice of the New York University (NYU) Spinal Cord Contusion system - IMPACTOR, was due to the fact that the Laboratory for Studies of Rachimedular Traumatism and Peripheral Nerves (LETRAN) is a participant in the Multicenter Animal Spinal Cord Injury Study (MASCIS), which permits the performance of comparable experimental studies
Summary
The potentially devastating effect of spinal cord injuries on the patient’s quality of life is a cause for concern among physicians that care for this kind of patient. The physiopathological changes caused by injury of the spinal cord affect multiple systems, while the extent of changes is related to the severity of the neurological damage. According to Holdsworth,[1] complete lesions are those in which there is no neurological function distal to the injury, and that can be reversible or irreversible. Nerve tissue injury occurs due to primary and secondary mechanisms. Primary injury is due to direct or indirect trauma at the level of the lesion; the mechanisms can be by flexion, extension, traction, compression, lateral inclination or laceration by a penetrating object, such as a firearm projectile.[2]
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