Abstract
Purpose: To study the radio-sanitization effect of paclitaxel liposomes on breast cancer cells, SK-BR-3.Methods: Breast cancer cell line SK-BR-3 was cultured and made into a cell suspension. Four groups of cells were used: control and radiotherapy groups, paclitaxel group, and paclitaxel liposome + radiotherapy group (combination group). The growth inhibitory effects of the different treatments on SKBR-3 cells were determined with CCK-8 method. Apoptosis in each group was evaluated with flow cytometry, while Western blotting was employed to assay Bcl-2, Caspase-3 and Bax protein levels.Results: There were marked inhibitory effect on growth of SK-BR-3 cells in drug, radiotherapy and combination groups, relative to control, while apoptosis was greater in combination group than in drug and radiotherapy groups (p < 0.05). The Bcl-2 protein level was higher in radiotherapy, drug and combination groups than in control group, while Caspase-3 and Bax proteins were markedly higher thancontrol values (p < 0.05).Conclusion: Paclitaxel liposomes exert radio-sensitization effect on SK-BR-3 by regulating the levels of apoptotic proteins. This provides a basis for research and development of new drugs.
 Keywords: Paclitaxel liposomes, Apoptotic proteins, Breast cancer, SK-BR-3, Radiotherapy, Sensitization, Bcl-2, Caspase-3, Bax
Highlights
Breast carcinoma is a frequently-occurring malignancy in women, accounting for more than 22 % of all female malignancies, and is a major factor involved in death of women
Inhibitory effect was markedly higher in combination group than in drug group and radiotherapy group
The growth inhibitory effect of the combination group was markedly higher than that of the drug group and radiotherapy group, while the drug group and radiotherapy group had no significant difference in the growth inhibitory effect on SK-BR-3 cells
Summary
Breast carcinoma is a frequently-occurring malignancy in women, accounting for more than 22 % of all female malignancies, and is a major factor involved in death of women. Paclitaxel is a yew extract with cytotoxic effects on many types of tumor cells. Karagiannis et al [5] reported that paclitaxel liposomes exert cytotoxic effects, and markedly reduce the adverse reactions of drugs. The present research was carried out to investigate radio-sensitizing effect of paclitaxel liposomes on SK-BR-3 cells, and the mechanism involved. The SK-BR-3 cells were product of Shanghai Supan Biotechnology Co. Ltd. Rabbit anti-human Caspase-3 monoclonal antibody was bought from Shanghai Ye Sheng Biotechnology Co. Ltd. Rabbit anti-human Bax polyclonal antibodies were obtained from Shanghai Xuan Ling Biological Technology Co. Ltd. The CCK-8 method was used to measure the growth inhibition in different treatment groups (control group, radiotherapy group, paclitaxel group and combination group). The supernatant was discarded, and apoptosis of the SK-BR-3 cells was measured flow cytometrically.
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