Abstract
Precolumn flow splitting is successfully implemented for gradient separation in peptide-mapping applications with a column internal diameter of 320 μm. Peptide standards, protein tryptic digests, and a crude peptide mixture from industry are used to demonstrate the feasibility for utilizing the packed-capillary LC/MS system in a variety of applications. High-sensitivity micro LC/MS peptide mapping and molecular weight determination can be performed routinely via ion-spray LC/MS with injected sample amounts as low as 10 pmol. Peptide sequence information is obtained via on-line packed-capillary LC/MS/MS
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