Abstract
SUMMARY In two distantly serologically related isolates (A and G12) of tomato black ring virus that each produce two RNA species, RNA-1 was found only in bottom component (B) particles and RNA-2 only in middle component (M) particles. Preparations of separated M and B particles were each barely infective, but produced 8 to 30 times more lesions when mixed, indicating that both kinds of particle are needed for infection, presumably because they contain different parts of the genome. Infectivity was not enhanced when M particles of isolate G12 were mixed with B particles of isolate A, but it was increased when M particles of isolate A were mixed with B particles of isolate G12. The lesions thus produced were abnormally small; isolates cultured from some of them were slower than the parental isolates to produce systemic symptoms in Chenopodium quinoa and had serological properties indicating that their coat protein cistron is in RNA-2. These properties were stable on subculture. Pseudo-recombinant isolates were also produced when RNA-2 of isolate A was mixed with RNA-1 of isolate G12, but not with the converse combination.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
