P85 Neurochemical signalling and participation of H2S and NO in fishes adult neurogenesis

Abstract

Fishes are an attractive animal model for investigation of the embryonal and postembryonal central nervous system development and participation in this processes some neurochemical agents. Pacific salmons Oncorhynchus masou, sturgeons Acipencer schrenckii and carps Cyprinus carpio were the objects of our investigation. The purpose of our investigation is to explore the participation of some neurotransmitters (dopamine, GABA), gasotransmitters (NO and H2S) and transcription factor Pax6 in the processes of post-embryonic neurogenesis in fish. In this work, we used methods of immunohistochemical labeling tyrosine hydroxylase, GABA, neuronal NOS, cystathionine β-synthase, transcription factor Pax6, PCNA. The area, which contained apoptosis have been diagnosed by TUNEL-labeling. Results of our research were established, that population of undifferentiated cells, which producing a OH, NOS, Pax6 on the territory of the matrix areas, containing proliferative PCNA-ir cells in the young O. masou 3-, 6-months and 1 year old was founded. The expression of Pax6 in the zones of proliferation, differentiation and migration in the brain integrative centers of adult carp and colocalization of Pax6, TH and CBS in the matrix zone of the brainstem, cerebellum and of lobus vagi. We argue that in periventricular proliferative zones identified expression tyrosine hydroxylase, GABA, NO and H2S, participating in the regulation of the basic histogenetic processes as morphogenetic factors, carrying out paracrinic and autocrinic regulation of cells-predecessors proliferation, migration of differentiating neurons and the expression of specific genes and synthesis.