P.6.c.006 The treatment of heroin withdrawal with dextromethorphan in Han Chinese: a randomized double-blind placebo-controlled trial

Abstract

We examined the spatial and temporal expression patterns of proteins and mRNAs of the components of transcription factor activator protein 1 (AP-1) to examine the activation pattern of lens epithelial cells during lens wound repair following an anterior capsular injury. One eye of adult Wistar rats (n = 106) were used. After making a lens anterior capsule incision with a hypodermic needle, the affected eye was enucleated 0 and 30 min, 1, 3, 5, 8, 10, 15, 20, 24 hr after injury. Forty six globes were processed for in situ hybridization with oligonucleotide probes for c-fos, fosB, c-jun, junB and junD mRNAs, and 60 globes were immunohistochemically analysed using anti-c-Fos and anti-c-Jun antibodies. Normal lens epithelial cells expressed mRNA signals forjunD , but not for c-fos, fosB, c-jun, and junB. mRNAs for c-fos,fosB , c-jun, and junB were detected in the whole lens epithelium from the vicinity to the wound to the equator from 30 min to 8 hr post-injury with their peaks after 30 min to 1 hr, but were no longer detected at 10 hr or later. Expression of c-fos mRNA in the equatorial lens cells was more marked than that ofc-jun mRNA.Immunohistochemistry showed that c-Fos protein was expressed in the lens epithelial cells in both the anterior and equatorial regions of the injured lens from 1 to 10 hr after injury, and was no longer detected at 12 hr. C-Jun protein was detected only in the equatorial lens cells from 1 to 5 hr after injury, and was no longer detected at 8 hr. Lens epithelial cells except those in the equatorial region did not express c-Jun protein. These findings indicate that transcriptional activation of lens epithelial cells is initiated in the very early phase after the lens injury, i.e. 30 min post-injury, suggesting that AP-1 may play important roles in regulating lens cell behavior during lens wound repair in rats.