Abstract
BackgroundUVB radiation is the main source of sunburn and skin cancers. Apoptosis eliminates photodamaged cells, and is thus important for preventing epidermal carcinogenesis. The cytoplasmic regulatory protein p62/A170/sequestosome 1 (p62) molecule is involved in a variety of cellular and signaling pathways. p62 is known to be and important in autophagy, but its role in UVB-induced apoptosis remains to be clarified. ObjectiveTo investigate the role of p62 against UVB-induced apoptotic changes, using mouse embryonic fibroblasts (MEFs) derived from p62 homozygous knockout (p62−/−) mice. Methodsp62−/− and wild-type (p62+/+) mice and MEFs were subjected to UVB irradiation, and the resultant apoptosis was analyzed using flow cytometry, quantitative real-time PCR, and western blots. ResultsApoptosis was decreased in the p62−/− MEFs compared to p62+/+ MEFs in response to UVB treatment. Compared with p62+/+ MEFs, p62−/− MEFs expressed significantly more Bcl-2 and less Bax, and showed increased Src and Stat3 phosphorylation. Our results show that p62 regulates apoptotic pathways by modifying critical signaling intermediates such as Src and Stat3. Conclusionp62 reduces UVB-induced apoptosis by modulating intrinsic apoptotic signaling through Src phosphorylation.
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