P5CR1 protein expression and the effect of gene-silencing on lung adenocarcinoma.

Yang She,Aiyou Mao,Xiaobin Wei,Feng Li

doi:10.7717/peerj.6934

Abstract

The present study aimed to investigate the expression of pyrroline-5-carboxylate reductase 1 (P5CR1) protein in lung adenocarcinoma and paracancerous tissues and to explore the effect of silencing the encoding gene PYCR1 on the proliferation, migration, invasion, and cisplatin sensitivity in lung adenocarcinoma cells, thereby providing a novel therapeutic target for the treatment of the disease. Immunohistochemistry staining was used to detect the P5CR1 protein expression in lung adenocarcinoma and paracancerous tissues, and statistical analysis evaluated the correlation between P5CR1 protein expression and gender, age, tissue part, or pathological grade. The CCK8 assay was performed to detect the proliferation and cisplatin sensitivity, while the effect of PYCR1 on the migration and invasion of lung adenocarcinoma cells was detected by scratch test and transwell chamber assay. The findings demonstrated that the P5CR1 protein expression was significantly elevated in lung adenocarcinoma tissues and correlated with the pathological grade, whereas no significant correlation was established between the protein expression and gender, age, or tissue part. Furthermore, after PYCR1 gene silencing, the proliferation and invasion were significantly suppressed, while the sensitivity to cisplatin was significantly enhanced. Therefore, it can be speculated that the PYCR1 gene affects the biological behavior of lung adenocarcinoma and cisplatin resistance, serving as a potential therapeutic target for lung adenocarcinoma.

Highlights

According to the Global cancer statistics 2012, lung cancer is the primary cause of cancer mortality, and non-small cell lung cancer (NSCLC) accounted for approximately 80–90% of lung cancer deaths worldwide (Torre et al, 2015)
pyrroline-5-carboxylate reductase 1 (P5CR1) protein expression in lung adenocarcinoma vs. paracancerous tissue and its correlation with clinical variables Immunohistochemistry detection demonstrated a significant increase in the expression of P5CR1 protein in 28 cases of lung adenocarcinoma tissues than that in 27 adjacent tissues (P < 0.001; Figs. 1A and 1B)
The results revealed that the expression of P5CR1 in lung adenocarcinoma was significantly higher in grade IIB-IIIB group than in grade IA-IIA group (P < 0.001), but no significant correlation was detected with gender, age, or tissue part (P > 0.05; Table 1)

Summary

Introduction

According to the Global cancer statistics 2012, lung cancer is the primary cause of cancer mortality, and non-small cell lung cancer (NSCLC) accounted for approximately 80–90% of lung cancer deaths worldwide (Torre et al, 2015). As an important pathological type of NSCLC, the incidence of lung adenocarcinoma is increasing year by year, and has even surpassed lung squamous cell carcinoma as the most important pathological type of NSCLC (Lortet-Tieulent et al, 2014). In China, approximately 0.3 million individuals are diagnosed with lung cancer, and of these about 0.25 million succumb to mortality annually (Chen et al, 2016). Lung cancer is associated with high incidence and mortality, causing heavy burden to the society. P5CR1 protein expression and the effect of gene-silencing on lung adenocarcinoma.

Methods

Results

Conclusion