P57-F Immune disfunction in acute professional stress

Abstract

Aim To study the state of the innate and adaptive components of immunity in military man in the acute phase of stress. Materials and methods The study included 27 men (mean age 27 ± 3.9 years) before and immediately after participation in elimination of emergency situations. Immune status was assessed by expression of CD 3+, CD 4+, CD 16+, CD 19+, CD 4 + CD25 + Foxp3, content granzyme in T- lymphocytes, NK cells in immunofluorescence test on a flow cytofluorimeter Cytomics FC 500 (Becman Coulter, USA) using appropriate monoclonal antibodies. Results The violation of differentiation with a significant decrease in the content of CD 4+ (51 ± 3.03% and 38 ± 2.6%) and increase the number of cytotoxic T lymphocytes (25.1 ± 3.13% and 36.8 ± 2.47%) were noted, their functional activation due to the increasing of the number of CD8 + containing Grasim (15.3 ± 3.73% and 28 ± 3.18%). There was a trend to reduction in the number of NK cells and their cytotoxic activity. The increase of the level of B-lymphocytes was not accompanied by changes of the basic classes of immunoglobulins. Conclusion The formation of the acute phase of stress in servicemen involved in military training accompanied by severe immune dysfunction.