Abstract
To clarify the association between the p53 polymorphism at codon 72 and susceptibility to the sporadic keratocystic odontogenic tumor (KCOT). One hundred KCOTs and 160 match-group healthy controls were genotyped to ascertain the frequency of the p53 codon 72 polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), confirmed by direct sequencing. The frequencies of the Pro/Pro, Arg/Pro, and Arg/Arg genotypes were 23.8%, 49.4%, and 26.9%, respectively, in the controls, while the KCOT cohort demonstrated 43.0%, 39.0%, and 18.0%, respectively. Further analysis suggested that p53 Pro could be a KCOT-susceptible allele (OR (95%CI)=1.77 (1.22 to 2.59), p=0.0024), with a sex-adjusted OR (95%CI) of 1.71 (1.17-2.50), p=0.0046. Moreover, the results indicated that p53 codon 72 Pro homozygous was associated with a two-fold risk of developing KCOT (adjusted OR (95%CI) =2.17(1.23-3.84), p=0.0062). The C/C genotype of P53 gene codon 72 increases the risk of developing sporadic KCOT and may be a useful tool for screening and diagnostic purposes.
Highlights
Keratocystic odontogenic tumor (KCOT) represents a common benign lesion of primarily odontogenic origin that arise from the dental lamina and its remnants
The results indicated that p53 codon 72 Pro homozygous was associated with a two-fold risk of developing keratocystic odontogenic tumor (KCOT) (adjusted ODDS ratio (OR) (95%confidence interval (CI)) =2.17(1.23-3.84), p=0.0062)
Genotyping of single nucleotide polymorphism (SNP): P53 codon 72 Paraffin-embedded KCOT samples and healthy control blood samples were used as specimen for genotyping
Summary
Keratocystic odontogenic tumor (KCOT) represents a common benign lesion of primarily odontogenic origin that arise from the dental lamina and its remnants. It is a rare and benign but develops into a locally aggressive cystic neoplasia. Work from our group has demonstrated that levels of methylation are essentially different in KCOT and ameloblastoma (Kitkumthorn and Mutirangura, 2010) These observed differences between the two sample groups were irrespective of clinical symptom and radiographic features which alone are largely inseparable. In this regard, a definitive diagnosis is essentially determined by a pathologist, after reviewing histopathological H&E stained tissue sections, subtle differences between KCOT samples may be missed
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