Abstract

Mutations or deletions in the tumor-suppressor gene p53 are among the commonest genetic changes found in human neoplasms including breast, lung and bowel cancers. In hematological malignancies, p53 is most often mutated in Burkitt's lymphoma, with p53 mutations present in 30 to 40% of tumor samples and in 70% of cell lines. To analyze the p53 gene alterations in child patients with B non-Hodgkin's lymphoma. Descriptive study. Tertiary oncology care center. The study investigated 12 patients with childhood B non-Hodgkin's lymphoma (Burkitt's lymphoma). Screening for p53 mutations was done by polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis of exon 5 to 8/9 of the gene. Abnormal polymerase chain reaction-single strand conformational polymorphism migration pattern was observed in 4 patients (33.3%), one on exon 6 and three on exon 7. Positive cases included 2 patients who died from disease. These preliminary results suggest that p53 mutations are quite frequent in children with Burkitt's lymphoma and may play a role in lymphoma genesis or disease progression.

Highlights

  • CONTEXT: Mutations or deletions in the tumorsuppressor gene p53 are among the commonest genetic changes found in human neoplasms including breast, lung and bowel cancers

  • Positive cases included 2 patients who died from disease. These preliminary results suggest that p53 mutations are quite frequent in children with Burkitt’s lymphoma and may play a role in lymphoma genesis or disease progression

  • Burkitt’s lymphoma is a subgroup of nonHodgkin’s lymphoma (NHL) with distinct epidemiological, clinical-pathological, immunological, and molecular features. It occurs as an Epstein-Barr virus associated with B nonHodgkin’s lymphoma with high incidence among children, in the malaria belt of equatorial Africa and sporadically in other geographical areas, when it is named sporadic Burkitt’s lymphoma.[5]

Read more

Summary

OBJECTIVE

To analyze the p53 gene alterations in child patients with B non-Hodgkin’s lymphoma. PARTICIPANTS: The study investigated 12 patients with childhood B non-Hodgkin’s lymphoma (Burkitt’s lymphoma). Screening for p53 mutations was done by polymerase chain reaction - single strand conformational polymorphism (PCR-SSCP) analysis of exon 5 to 8/9 of the gene

INTRODUCTION
METHODS
RESULTS
DISCUSSION
11. The non-Hodgkin’s Lymphoma Pathologic Classification Project
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call