Abstract
The aim of this study was to characterize and compare Ca2+dependent electrical propagation in cell cultures showing a continuous structure, an isotropic structure with a pivot point and a highly anisotropic structure. Ventricular cells of neonatal rat hearts were grown on coverslips using the technique of patterned growth. The growth pattern either consisted of (i) linear strands (width 300μm), (ii) an isotropic growth area with an obstacle ending with a pivot point (width 50μm), or (iii) a highly anisotropic structure with single parallel strands (width 70μm, interstrand distance 30μm) interconnected at intervals of 1mm.
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