P420 GALLIC ACID HAS ANTIVIRAL EFFECT AGAINST HEPATITIS C VIRUS (HCV), WHICH IS MEDIATED BY ITS ANTIOXIDANT ACTIVITY

Abstract

growth factor (HGF) plays a key role in liver regeneration. It is determined that HGF concentration is increased on the second day after the common bile duct ligation. In the same time, previously we have shown significant increasing of mitotic activity and polyploidization on 4th day of cholestasis. The aim of this work is to determine whether the HGF signaling pathway initiates hepatocytes polyploidization. Methods: Experimental animals: white rats (130–150g). Model of cholestatic liver: common bile duct ligation. C-Met inhibitor (PHA665752) was used for blocking HGF signaling pathway. Animals groups: I-control, II-cholestatic animals (4th day), IIIcholestatic animals with inhibitor injection (1mg/kg) during 4 days after surgery. Proliferative activity: colchicine mitotic index. DNA content of hepatocytes, stained by Schiff reagent, was detected by using of computer software ImageJ 1.36b. Results: It was established that quantity of polyploid cells (4c, 4cx2, 8c) was increased in II group in compare to intact animals. Injections of inhibitor have not influence on the proportion of polyploid cells in animals of III group. At the same time, hepatocytes mitotic index is decreased in the III group compared to II group. Conclusions: From obtained results we can conclude that HGF signaling pathway doesn’t serve as an activator for the mechanism of polyploidization in cholestatic liver.