Abstract
p40 phox activated phagocyte NADPH oxidase without p47 phox in a cell-free system consisting of p67 phox, Rac and cytochrome b 558 relipidated with phosphatidylinositol 3-phosphate. The activation reached to 70% of that by p47 phox. Addition of p47 phox slightly increased the activation, but not additively. p40 phox improved the efficiency of p67 phox in the activation. The C-terminus-truncated p67 phox, p40 phox(D289A), p40 phox(R58A), or p40 phox(W207R) showed an impaired activation. A peptide corresponding to the p22 phox Pro-rich region suppressed the activation, and far-western blotting revealed its interaction with p40 phox SH3 domain. Thus, p40 phox can substitute for p47 phox in the activation, interacting with p22 phox and p67 phox through their specific regions.
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