P-397: Zinc protects the lead induced testicular damage in albino rats

Abstract

ObjectiveWith increasing concern about the environmental pollution, the interaction of micronutrients with toxic metals is of great interest. In this study, the testicular effects of lead following pretreatment with zinc were observed. The lead inhibits the activity of superoxide dismutase and catalase. Superoxide dismutase is necessary for conversion of toxic Reactive Oxygen species into inert products, which will then excrete from the cells. The superoxide dismutase is a zinc dependent enzyme, restored with zinc supplementationDesignExperimental study.Materials and methodsSixty adult male albino rats were selected for study. The albino rats were divided into three groups each comprises of 20 animals. The group A, served as control group, received injection normal saline 1 cc interaperitonealy daily for 1, 3, 5 and 8 weeks. Group B, received the injection lead chloride in a dose of 10 mg/ kg body weight interaperitonealy daily for their respective period of treatment. Group C, received the injection lead chloride in a dose of 10 mg/ kg body weight and zinc chloride in a dose of 1 mg/ kg interaperitonealy daily for their respective period of treatment. The animals were sacrificed on the day of completion of treatment. The testes along with epididymis were removed; the epididymis was used for semen analysis. The testes were fixed in Bouin’s fluid for 24 hours. The testes were processed, the paraffin blocks were made. 4 μm tissue sections were made, stained with PAS and Iron Heamatoxyline method and the morphometric study was done. The results were compared with control and correlated with the previous studies.ResultsThe absolute and relative weights of Testes of group B decreased significantly (P< 0.001) as compare to groups A and C. The numbers of spermatozoa in animals of group B was decreased significantly (P< 0.001) as compare to groups A and C. The immotility of spermatozoa of animals of group B was significantly (P<0.001) increased as compare to groups A and C.ConclusionBased on the study, lead chloride induced toxicity on testes and the pretreatment with zinc chloride in albino rats could ameliorate the testicular damage. ObjectiveWith increasing concern about the environmental pollution, the interaction of micronutrients with toxic metals is of great interest. In this study, the testicular effects of lead following pretreatment with zinc were observed. The lead inhibits the activity of superoxide dismutase and catalase. Superoxide dismutase is necessary for conversion of toxic Reactive Oxygen species into inert products, which will then excrete from the cells. The superoxide dismutase is a zinc dependent enzyme, restored with zinc supplementation With increasing concern about the environmental pollution, the interaction of micronutrients with toxic metals is of great interest. In this study, the testicular effects of lead following pretreatment with zinc were observed. The lead inhibits the activity of superoxide dismutase and catalase. Superoxide dismutase is necessary for conversion of toxic Reactive Oxygen species into inert products, which will then excrete from the cells. The superoxide dismutase is a zinc dependent enzyme, restored with zinc supplementation DesignExperimental study. Experimental study. Materials and methodsSixty adult male albino rats were selected for study. The albino rats were divided into three groups each comprises of 20 animals. The group A, served as control group, received injection normal saline 1 cc interaperitonealy daily for 1, 3, 5 and 8 weeks. Group B, received the injection lead chloride in a dose of 10 mg/ kg body weight interaperitonealy daily for their respective period of treatment. Group C, received the injection lead chloride in a dose of 10 mg/ kg body weight and zinc chloride in a dose of 1 mg/ kg interaperitonealy daily for their respective period of treatment. The animals were sacrificed on the day of completion of treatment. The testes along with epididymis were removed; the epididymis was used for semen analysis. The testes were fixed in Bouin’s fluid for 24 hours. The testes were processed, the paraffin blocks were made. 4 μm tissue sections were made, stained with PAS and Iron Heamatoxyline method and the morphometric study was done. The results were compared with control and correlated with the previous studies. Sixty adult male albino rats were selected for study. The albino rats were divided into three groups each comprises of 20 animals. The group A, served as control group, received injection normal saline 1 cc interaperitonealy daily for 1, 3, 5 and 8 weeks. Group B, received the injection lead chloride in a dose of 10 mg/ kg body weight interaperitonealy daily for their respective period of treatment. Group C, received the injection lead chloride in a dose of 10 mg/ kg body weight and zinc chloride in a dose of 1 mg/ kg interaperitonealy daily for their respective period of treatment. The animals were sacrificed on the day of completion of treatment. The testes along with epididymis were removed; the epididymis was used for semen analysis. The testes were fixed in Bouin’s fluid for 24 hours. The testes were processed, the paraffin blocks were made. 4 μm tissue sections were made, stained with PAS and Iron Heamatoxyline method and the morphometric study was done. The results were compared with control and correlated with the previous studies. ResultsThe absolute and relative weights of Testes of group B decreased significantly (P< 0.001) as compare to groups A and C. The numbers of spermatozoa in animals of group B was decreased significantly (P< 0.001) as compare to groups A and C. The immotility of spermatozoa of animals of group B was significantly (P<0.001) increased as compare to groups A and C. The absolute and relative weights of Testes of group B decreased significantly (P< 0.001) as compare to groups A and C. The numbers of spermatozoa in animals of group B was decreased significantly (P< 0.001) as compare to groups A and C. The immotility of spermatozoa of animals of group B was significantly (P<0.001) increased as compare to groups A and C. ConclusionBased on the study, lead chloride induced toxicity on testes and the pretreatment with zinc chloride in albino rats could ameliorate the testicular damage. Based on the study, lead chloride induced toxicity on testes and the pretreatment with zinc chloride in albino rats could ameliorate the testicular damage.