P-378 - Sleeve gastrectomy improves endothelial dysfunction in type 2 diabetic rats

Abstract

The aim of this study was to investigate the effects of sleeve gastrectomy bariatric surgery on aorta artery function. Diabetic Goto-Kakizaki (GK) rats were divided in four experimental groups and subjected to specific diets for five months (normal or high fat diets (HFD) and compared with normal Wistar rats. The effects of sleeve gastrectomy, sham surgery and HFD diets were investigated on NO-dependent vasorelaxation in isolated rat aortic arteries from the different groups. Lipid profile, glucose tolerance, NO bioavailability and vascular oxidative stress were also evaluated. Sleeve gastrectomy significantly ameliorated NO-dependent vasorelaxation in HFD-fed GK rats while sham surgery had no effects. Endothelial dysfunction in HFD GK rats was accompanied by a decrement in NO bioavailability and by a significantly increment in vascular oxidative stress in HFD treated GK rats and bariatric surgery was able to ameliorate both parameters. Bariatric surgery and its attendant improvements in weight, oxidative stress and metabolism collectively reverse the obesity-induced aggravation of endothelial dysfunction in diabetic GK rats. This effect is due to a significant decrement in vascular oxidative stress and an improvement in nitric oxide bioavailability.