P36 Proteomics studies on effect of Xiao-Xu-Ming decoction on cerebral ischemia rat by UPLC-Q-Exactive technology

Abstract

Xiao-xu-ming decoction (XXMD) is one of the classic traditional Chinese medicine prescriptions. Our studies demonstrated the anti-ischemic effect of XXMD evidenced by protecting the physical membrane barrier system (permeability and tight junction protein ZO-1, occludin and claudin-5) and efflux transporter system (especially including P-GP transporter, BCRP transporter and MRP2 transporter) of blood-brain barrier, and regulating the mitochondrial proteome via the PKA and PKC signalling pathway in brain endothelial cells. The effect of XXMD on cerebral ischemia in the rat was investigated and a proteomics method based on UPLC-Q-Exactive applied to explore the biological basis of any observed effect of XXMD. The cerebral ischemia rat model was established using nylon wire to induce 2 h of cerebral ischemia in healthy male SD rats. Proteins of pallium from the blank group, the model group and the XXMD group were detected by UPLC-Q-Exactive system. The data was imported into Proteome Discoverer and Mascot software to identify and analyze all the proteins. Compared with the model group, 702 significantly different proteins were found in the XXMD group, and 789 significantly different proteins were found in the blank group. Through the biological function analysis, these proteins could be classified into cellular process, biological regulation, metabolic process, response to stimulus, cellular component organization or biogenesis, or immune system process. The above target proteins and their regulation pathways may be the biological basis via which XXMD plays an important role in cerebral ischemia. Acknowledgements Project supported by National Natural Science Foundation of China (81473579, 81273654 and 81102879), Beijing Natural Science Foundation (7173267), and National Science and Technology Major Projects (2013ZX09103002-022).