P358 Candida auris survival on common medical supply surfaces under different environmental conditions

Abstract

Poster session 3, September 23, 2022, 12:30 PM - 1:30 PMBackground Candida auris is an emerging multidrug-resistant pathogenic yeast. The increasing frequency of C. auris outbreaks is prompting alarm worldwide. This yeast survives and spreads on contaminated medical supplies, resulting in hospital outbreaks. To learn more about the yeast's spreading behaviors and transmission, we studied its persistence and survival on a variety of medical-related surfaces under diverse environmental conditions.MethodsA total of 104 CFU/mL solutions of four Candida species, including C. auris, C. albicans, C. parapsilosis, and C. glabrata, were inoculated onto different 2 × 2 cm sheets of cotton textile, polystyrene, paper, aluminum, glass, latex, and dried Sabouraud dextrose agar. Inoculated sheets were incubated at various temperatures and subjected to light and darkness at 1, 2, 7, 14, 30, 45, 60, and 120-day intervals. After culture of the sheets on Sabouraud dextrose agar plates, the viable CFUs of yeasts were counted.ResultsAll four species remained alive on all surfaces for at least 1 week under ambient and refrigerator temperatures, darkness, and light exposure. However, only latex and polystyrene surfaces maintained viable C. auris and C.parapsilosis for a maximum of 30 days at ambient temperature and darkness. C. auris survived on dried Sabouraud dextrose agar sheets for ˃4 months.Conclusions Candida auris and other pathogenic yeasts can survive on a variety of medical surfaces for extended periods of time. Latex and polystyrene devices are the best medical matrices for yeast persistence. If C. auris has access to organic and nutritional components, its survival could be greatly increased. To prevent C. auris transmission, appropriate disinfection and decontamination methods should be considered.